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基质辅助飞行的完整蛋白质(MALDI-TOF)质谱分析激光解吸/电离飞行时间大于100 kDa的
JoVE Journal
Química
This content is Free Access.
JoVE Journal Química
Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
DOI:

07:49 min

September 09, 2013

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Capítulos

  • 00:05Título
  • 01:09Recrystallization of Matrices in order to Improve Their Purity (Optional)
  • 02:06Cleaning of MALDI Stainless Steel Target
  • 02:49&#945-CHCA Thin Layer Solution: Preparation and Deposition on the MALDI Target
  • 03:18Preparation of Matrix Solutions: &#945-CHCA, DHB, and CHCA_DHB Mixture
  • 04:04Sample Deposition on the MALDI Target and MALDI Spectra Acquisition
  • 05:17Results: MALDI-TOF Analysis of Proteins Larger than 100 kDa
  • 07:08Conclusion

Summary

Tadução automática

精确质量测定代表蛋白质的调查过程中的重要一步。基质辅助激光解吸/电离(MALDI)质谱(MS)可用于这种确定和其主要优点是耐受的盐,去垢剂和污染物。在这里,我们示出了用于蛋白质大于100 kDa的通过MALDI-MS较大的分析可访问的方法。

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