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Imunologia e Infecção
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Culturing Microglia from the Neonatal and Adult Central Nervous System
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Culturing Microglia from the Neonatal and Adult Central Nervous System

Culturing Microglia from the Neonatal and Adult Central Nervous System

DOI:
10.3791/50647-v

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11:28 min

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August 09, 2013

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, , ,
1Program in Neuroscience,Stony Brook University, 2Department of Pharmacological Sciences,Stony Brook University, 3Program in Molecular and Cellular Pharmacology,Stony Brook University

Capítulos

  • 00:05Título
  • 01:20Neonatal Microglia Dissection: Day 0
  • 04:24Cell Culture: Day 0
  • 05:31Cell Culture: Day 10
  • 06:40Adult Microglia: Tissue Colection
  • 07:56Digestion of Spinal Cord Tissue
  • 09:07Results: Representative Images of Neonatal and Adult Microglia
  • 10:50Conclusion

Summary

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We outline methods for the efficient and quick isolation/culture of viable microglia from the neonatal cerebral cortex and adult spinal cord. The dissection and plating of cortical microglia can be accomplished within 90 minutes, with the subsequent microglial harvest taking place ~ 10 days following the initial dissection.

Tags

Keywords: MicrogliaCNSCentral Nervous SystemMacrophageNeonatalAdultMultiple SclerosisSpinal Cord InjuryAxonal DamageIschemic Brain TraumaStrokeSynaptic PlasticityCell CultureAdult CellsActivationPathologyIn Vitro

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