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Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates

Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates

DOI:
10.3791/50665-v

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10:44 min

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November 18, 2013

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, ,
1Department of Comparative Biology & Experimental Medicine, Faculty of Veterinary Medicine,University of Calgary

Capítulos

  • 00:05Título
  • 01:30Preparing Microwells to Receive Cells
  • 02:58Preparing Cells
  • 04:48Spheroid Formation
  • 08:50Results: Spheroid Harvest
  • 10:14Conclusion

Summary

Tadução automática

Tadução automática

We introduce a protocol for the generation of large numbers (thousands to hundreds of thousands) of uniform size- and composition-controlled tumor spheroids, using commercially available microwell plates.

Tags

Keywords: Tumor Spheroids3D Cell CultureMicrowell PlatesAggreWellHigh-throughputReproducibilityCell AggregationTumor BiologyTherapeutic Screening

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