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Imunologia e Infecção
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In vivo Imaging Method to Distinguish Acute and Chronic Inflammation
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In vivo Imaging Method to Distinguish Acute and Chronic Inflammation

In vivo Imaging Method to Distinguish Acute and Chronic Inflammation

DOI:
10.3791/50690-v

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07:10 min

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August 16, 2013

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,
1Lurie Family Imaging Center, Dana-Farber Cancer Institute,Harvard Medical School, 2Division of Pediatric Hematology/Oncology/Stem Cell Transplantation,Columbia University Medical Center

Capítulos

  • 00:05Título
  • 01:55Subcutaneous PMA Inflammation Model
  • 02:58Bioluminescence Imaging of Inflammation
  • 05:11Results: Longitudinal Bioluminescence Imaging of Inflammation
  • 06:39Conclusion

Summary

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We describe a non-invasive imaging method for distinguishing inflammatory stages. Systemic delivery of luminol reveals areas of acute inflammation dependent upon MPO activity in neutrophils. In contrast, injection of lucigenin allows for visualization of chronic inflammation dependent upon Phox activity in macrophages.

Tags

InflammationAcute InflammationChronic InflammationNeutrophilsMacrophagesBioluminescence ImagingLuminolLucigeninMyeloperoxidase (MPO)Phagocyte NADPH Oxidase (Phox)Non-invasive ImagingPathogen Invasion

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