Journal
/
Biologia
/
Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

DOI:
10.3791/50692-v

•

06:39 min

•

October 04, 2013

•

, ,
1Vascular Biology Program, Department of Surgery,Boston Children’s Hospital and Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering,Harvard University, 3Harvard School of Engineering and Applied Sciences

Capítulos

  • 00:05Título
  • 01:16Pre-operative Preparation
  • 02:48Intraductal Injection and Analysis of Mammary Gland Tissue
  • 05:02Results: Mammary Gland Injections Enable Localized Delivery of Molecules
  • 06:15Conclusion

Summary

Tadução automática

Tadução automática

A protocol for the non-invasive intraductal delivery of aqueous reagents to the mouse mammary gland is described. The method takes advantage of localized injection into the nipples of mammary glands targeting mammary ducts specifically. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents and small molecules.

Tags

Keywords: Intraductal InjectionLocalized Drug DeliveryMouse Mammary GlandEvans BlueFluorescent ReagentsNon-invasiveSiRNAChemotherapeutic AgentsSmall Molecules

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/50692?article_type=v

Mouse Mammary Epithelial Cells form Mammospheres During Lactogenic Differentiation
check_url/pt/50692?article_type=v

The Microfluidic Probe: Operation and Use for Localized Surface Processing
check_url/pt/50692?article_type=v

Changes in Mammary Gland Morphology and Breast Cancer Risk in Rats
check_url/pt/50692?article_type=v

Evaluation of Mammary Gland Development and Function in Mouse Models
check_url/pt/50692?article_type=v

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
check_url/pt/50692?article_type=v

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
check_url/pt/50692?article_type=v

A Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection
check_url/pt/50692?article_type=v

Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology
check_url/pt/50692?article_type=v

Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis
check_url/pt/50692?article_type=v

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Read Article