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Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System
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Biologia
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JoVE Journal Biologia
Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System

Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System

DOI:
10.3791/50717-v

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16:41 min

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November 05, 2013

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,
1Department of Biochemistry,University of Geneva

Capítulos

  • 00:05Título
  • 02:42OxyBurst Green (OBG)-Coating of Beads
  • 05:43Visualization and Qualitative Measurement of ROS Production in Phagosomes
  • 08:48Quantitative and Medium-throughput Measurement of Intracellular Superoxide Production
  • 10:30Quantitative and Medium-throughput Measurement of Extracellular H2O2 Production
  • 12:04Results: OxyBurst Green (OBG), Dihydroethidium (DHE) and Amplex UltraRed (AUR) Assays can Detect and Measure ROS in Dictyostelium
  • 15:54Conclusion

Summary

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We adapted a set of protocols for the measurement of reactive oxygen species (ROS) that can be applied in various amoeba and mammalian cellular models for qualitative and quantitative studies.

Tags

Reactive Oxygen SpeciesROSOxyBurst GreenDihydroethidiumDHEAmplex UltraRedAURDictyosteliumPhagocytesLipopolysaccharideLPSSuperoxideHydrogen PeroxideFluorescence MicroscopyQuantitation

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