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Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
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Biologia
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JoVE Journal Biologia
Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

DOI:
10.3791/50733-v

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09:38 min

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November 26, 2013

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, ,
1University of Victoria-Genome BC Proteomics Centre,University of Victoria, 2Department of Biochemistry and Microbiology,University of Victoria

Capítulos

  • 00:05Título
  • 01:06Tissue Sectioning
  • 02:28Matrix Coating
  • 03:10MALDI MS
  • 06:37Data Analysis
  • 07:38Results: Bovine Calf Lens Tissue
  • 09:05Conclusion

Summary

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Tadução automática

Dithranol (DT; 1,8-dihydroxy-9,10-dihydroanthracen-9-one) has previously been reported as a MALDI matrix for tissue imaging of small molecules; protocols for the use of DT for the MALDI imaging of endogenous lipids on the surface of tissue sections by positive-ion MALDI-MS on an ultrahigh-resolution quadrupole-FTICR instrument are provided here.

Tags

Keywords: MALDI ImagingDithranol MatrixFTICR Mass SpectrometryLow-molecular Weight CompoundsEndogenous Lipids

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