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Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose
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Biologia
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JoVE Journal Biologia
Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose

Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose

DOI:
10.3791/50734-v

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12:11 min

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November 13, 2013

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, ,
1Department of Bioorganic Chemistry,Max Planck Institute for Chemical Ecology

Capítulos

  • 00:05Título
  • 02:3513C-Labeling of DNA in Metabolically Active Gut Bacteria
  • 03:42Insect Dissection
  • 05:06DNA Extraction and Amplification
  • 06:13CsCl Gradient/DNA Ultracentrifugation
  • 08:27Processing the CsCl Gradients
  • 09:45Identifying Metabolically Active Gut Bacteria
  • 11:13Conclusion

Summary

Tadução automática

Tadução automática

The active bacterial community associated with the gut of Spodoptera littoralis, was determined by stable-isotope-probing (SIP) coupled to pyrosequencing. Using this methodology, identification of the metabolically active bacteria species within the community was done with high resolution and precision.

Tags

Keywords: Stable Isotope Probing13C-glucoseMetabolically Active BacteriaSpodoptera LittoralisInsect GutMicrobial CommunityPyrosequencingCulture-independent TechniquesDNA Density Gradient Centrifugation

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