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Capítulos
- 00:05Título
- 01:28Isolation of the Superior Epigastric Artery (SEA)
- 04:24Isolation of the Endothelial Tube
- 08:08Results: Representative Calcium Fluorescence in an Endothelial Cell Tube
- 08:46Conclusion
We present a preparation for visualizing and manipulating calcium signaling in native, intact microvascular endothelium. Endothelial tubes freshly isolated from mouse resistance arteries supplying skeletal muscle retain in vivo morphology and dynamic signaling within and between neighboring cells. Endothelial tubes can be prepared from microvessels of other tissues and organs.
