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Protokoller for å gjennomføre en<em> Escherichia coli</em> Basert TX-TL Cell-Free Expression System for syntetisk biologi
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Biologia
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JoVE Journal Biologia
Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Protokoller for å gjennomføre en<em> Escherichia coli</em> Basert TX-TL Cell-Free Expression System for syntetisk biologi

DOI:
10.3791/50762-v

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16:11 min

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September 16, 2013

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, , , , ,
1Department of Biology,California Institute of Technology, 2Department of Bioengineering,California Institute of Technology, 3Synthetic Biology Center, Department of Bioengineering,Massachusetts Institute of Technology, 4School of Physics and Astronomy,University of Minnesota

Capítulos

  • 00:05Título
  • 01:41Cell Lysis
  • 06:29Extract Clarification
  • 08:32Experimental Execution of a TX-TL Reaction
  • 10:40Results: Assessment of TX-TL Cell-free Expression System
  • 15:56Conclusion

Summary

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Tadução automática

Denne fem-dagers protokollen skisserer alle trinn, utstyr, og supplerende programvare som er nødvendig for å skape og drive en effektiv endogen Escherichia coli basert TX-TL celle-fri ytring system fra bunnen av. Med reagenser, tar protokollen 8 timer eller mindre å sette opp en reaksjon, samle inn og behandle data.

Tags

Keywords: Cell-free Expression SystemEscherichia ColiTX-TLTranscription-translationSynthetic BiologyProtein ExpressionIn VitroEndogenousT7 RNA PolymeraseLac/tet RepressorsMRNA DegradationDNA DegradationBiomolecular Breadboard

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