Journal
/
Medicina
/
Revealing the Cytoskeletal Organization of Invasive Cancer Cells in 3D
JoVE Journal
Medicina
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Medicina
Revealing the Cytoskeletal Organization of Invasive Cancer Cells in 3D

Revealing the Cytoskeletal Organization of Invasive Cancer Cells in 3D

DOI:
10.3791/50763-v

•

11:09 min

•

October 26, 2013

•

, ,
1UMR 144 CNRS,Institut Curie

Capítulos

  • 00:05Título
  • 01:48TAMRA-collagen I Labeling
  • 03:453D TAMRA-collagen Matrices with Embedded Single Cells
  • 05:133D TAMRA-collagen Matrices with Embedded Cell Spheroids
  • 06:343D Immunofluorescence Staining
  • 07:45Sample Imaging
  • 08:37Results: Representative 3D Immunofluorescent Images
  • 10:36Conclusion

Summary

Tadução automática

Tadução automática

This article presents a method to fluorescently label collagen that can be further used for both fix and live imaging of 3D cell cultures. We also provide an optimized protocol to visualize endogenous cytoskeletal proteins of cells cultured in 3D environments.

Tags

3D Cell CultureCell MigrationCancer CellsCytoskeletonCollagen I3D ImmunofluorescenceConfocal Microscopy

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice

Three-Dimensional (3D) Tumor Spheroid Invasion Assay

Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots

Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model

A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting

Minimally Invasive Transverse Aortic Constriction in Mice

Read Article