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A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome
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Biologia
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JoVE Journal Biologia
A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome

A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome

DOI:
10.3791/50773-v

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06:33 min

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December 09, 2013

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, , ,
1Université Claude Bernard Lyon, 2Centre de Génétique et de Physiologie Moléculaires et Cellulaires,CNRS UMR 5534

Capítulos

  • 00:05Título
  • 01:11Culturing Transgenic Nematodes Prior to Integration
  • 02:38UV Irradiation and Recovery of Transgenic Worms
  • 03:18Isolation of the Integrated Transgenic Lines
  • 04:59Results: Rapid Integration of Transgenes
  • 06:01Conclusion

Summary

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Tadução automática

This protocol describes a rapid and low material consuming procedure for the integration of transgenic extrachromosomal arrays into the Caenorhabditis elegans genome using ultra violet (UV) irradiation. Furthermore, this protocol is particularly well suited for transgenic lines that transmit extrachromosomal arrays at a high rate.

Tags

C. ElegansExtrachromosomal ArraysTransgenic LinesUV IrradiationGenome IntegrationHigh Transmission RateMendelian SegregationNonmosaic ExpressionRapid ProtocolTransgene Expression

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