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Udarbejdelse af Primary neuroner til visualisering neurites i frossen hydreret tilstand vha. Cryo-Electron Tomography
 
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Udarbejdelse af Primary neuroner til visualisering neurites i frossen hydreret tilstand vha. Cryo-Electron Tomography

Article DOI: 10.3791/50783-v • 09:59 min • February 12th, 2014
February 12th, 2014 •
1, 2, 3, 4, 2, 3, 4
1Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 2Department of Neuroscience, Baylor College of Medicine, 3Department of Neuroscience, University of California at San Diego, 4National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine

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For at bevare neuronale processer til ultrastrukturelle analyse beskriver vi en protokol til galvanisering af primære neuroner på elektronmikroskopi gitre efterfulgt af flash frysning, hvilket giver prøver suspenderet i et lag af glasagtig is. Disse prøver kan undersøges med en cryo-elektron mikroskop til at visualisere strukturer på nanometer skala.

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Neuroscience neuroner Cryo-elektronmikroskopi Electron Microscope Tomography Brain rotte primær neuron kultur morfologisk analyse
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