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MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
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Biologia
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JoVE Journal Biologia
MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

DOI:
10.3791/50785-v

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12:21 min

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November 30, 2013

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,
1Department of Physiology,Medical College of Wisconsin

Capítulos

  • 00:05Título
  • 01:20Tissue Preparation
  • 03:28Hybridization
  • 05:29Stringency Wash
  • 06:20Detection
  • 07:48Mounting Slides
  • 08:26Results: In situ Hybridization Detects miRNA in Kidney Tissues
  • 11:42Conclusion

Summary

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This article describes an in situ hybridization protocol optimized for colormetric detection of microRNA expression in formalin fixed kidney sections.

Tags

MiRNAIn Situ HybridizationMicroRNA DetectionProbe ConcentrationIncubation ConditionsColorimetric Detection

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