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微RNA<em>原位</em>杂交的福尔马林固定的肾组织

JoVE Journal
Biologia

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JoVE Journal Biologia

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

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微RNA<em>原位</em>杂交的福尔马林固定的肾组织

DOI:

10.3791/50785-v

•

12:21 min

•

November 30, 2013

•

Alison J. Kriegel, Mingyu Liang

¹Department of Physiology,Medical College of Wisconsin

Capítulos

00:05Título
01:20Tissue Preparation
03:28Hybridization
05:29Stringency Wash
06:20Detection
07:48Mounting Slides
08:26Results: In situ Hybridization Detects miRNA in Kidney Tissues
11:42Conclusion

Summary

Tadução automática

本文介绍了比色法检测福尔马林固定的肾部分microRNA表达谱进行优化的原位杂交协议。

Tags

Keywords: MiRNA In Situ Hybridization MicroRNA Detection Probe Concentration Incubation Conditions Colorimetric Detection

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