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Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo
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Capítulos
- 00:05Título
- 01:29Fixation of Protein-protein Interactions by Formaldehyde Cross-linking in Living Cells
- 04:07Purification of Strep-tagged Membrane Protein
- 05:24Purification of Strep-tag Membrane Bait Protein and SDS-PAGE
- 07:43Confirmation of Interaction Partners
- 08:53Results: Membrane SPINE Analysis
- 10:33Conclusion
A biochemical approach is described to identify in vivo protein-protein interactions (PPI) of membrane proteins. The method combines protein cross-linking, affinity purification and mass spectrometry, and is adaptable to almost any cell type or organism. With this approach, even the identification of transient PPIs becomes possible.
