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幻灯片制备方法，以保存睾丸细菌细胞的三维染色质结构
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Biologia
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JoVE Journal Biologia
Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells
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幻灯片制备方法，以保存睾丸细菌细胞的三维染色质结构

DOI:
10.3791/50819-v

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07:34 min

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January 10, 2014

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1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine

Capítulos

  • 00:05Título
  • 01:543D Slide Preparation
  • 04:03Cot-1 RNA FISH
  • 06:08Results: 3D Chromatin Architecture
  • 07:09Conclusion

Summary

Tadução automática

Tadução automática

这里的材料描述了一种为保存睾丸生殖细胞的三维染色质结构而开发的方法。这被称为三维（3D）滑动方法。该方法提高了对亚核结构检测的灵敏度，适用于 原位 杂交（FISH）中的免疫荧光、DNA和RNA荧光。

Tags

Three-dimensional Chromatin ArchitectureTesticular Germ CellsSlide Preparation MethodPermeabilizationFixationCytospinImmunofluorescenceDNA FISHRNA FISHCot-1 RNA FISHGerm Cell Differentiation

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