É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:08Embryo Dissection
- 02:07Embryo Slicing
- 04:14Mandible Slice Culture and Lineage Tracing
- 06:47Documenting Early Stages of Tooth Development
- 07:22Conclusion
Here we detail a method to culture tooth germs in mandible slices using a tissue chopper. This method allows unique access to the tooth during development, providing excellent opportunity for manipulation and lineage tracing, not available using more traditional culture methods.
