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Rescate del virus de la enfermedad de Newcastle recombinante a partir de cDNA
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1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Global Health and Emerging Pathogens Institute,Icahn School of Medicine at Mount Sinai, 3Department of Medicine,Icahn School of Medicine at Mount Sinai, 4Department of Microbiology and Immunology, School of Medicine and Dentistry,University of Rochester
Capítulos
- 00:05Título
- 01:37Infection of Mammalian Cells with the Recombinant Modified Vaccinia Ankara Virus Expressing the Bacteriophage T7 RNA Polymerase (MVA-T7)
- 02:40Transfection of Mammalian Cells
- 03:48Co-culture of Mammalian Cells with Avian Cells
- 05:22Infection of Chicken Embryonated Eggs
- 06:31Harvest of Allentoic Fluid from Infected Chicken Embryonated Eggs
- 07:52Hemagglutination (HA) Assay
- 09:03Results: Transfection and MVA-T7 Infection Efficiencies and Hemagglutination Assay
- 10:33Conclusion
Virus de la enfermedad de Newcastle (NDV) se ha estudiado ampliamente en los últimos años con el fin de desarrollar nuevos vectores para la vacunación y la terapia, entre otros. Estos estudios han sido posible debido a las técnicas para rescatar virus recombinante a partir de cDNA, tales como las que se describen aquí.
