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Organel Transport i dyrkede<em> Drosophila</em> Celler: S2 Cell Line og Primary neuroner.
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Biologia
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JoVE Journal Biologia
Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

Organel Transport i dyrkede<em> Drosophila</em> Celler: S2 Cell Line og Primary neuroner.

DOI:
10.3791/50838-v

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10:08 min

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November 20, 2013

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, ,
1Department of Cell and Molecular Biology,Feinberg School of Medicine, Northwestern University, 2IKERBASQUE,Basque Foundation for Science

Capítulos

  • 00:05Título
  • 01:34Drosophila S2 Cells
  • 03:19Drosophila Neuron Culture
  • 07:47Visualized Microtuble Transport in Cultured S2 Cells and Neurons
  • 09:42Conclusion

Summary

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Tadução automática

Drosophila S2-celler og dyrkede neuroner er gode systemer til billeddannelse af motordreven organel transport in vivo. Her beskriver vi detaljerede protokoller til dyrkning både celletyper, deres billedbehandling og analyse af transport.

Tags

DrosophilaS2 CellsPrimary NeuronsOrganelle TransportMicrotubulesLive ImagingRNAiCargo TransportAxonal TransportCell Culture

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