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High Throughput Microinjections af søpindsvin Zygoter
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Biologia
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JoVE Journal Biologia
High Throughput Microinjections of Sea Urchin Zygotes

High Throughput Microinjections af søpindsvin Zygoter

DOI:
10.3791/50841-v

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12:40 min

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January 21, 2014

•

,
1Department of Biological Sciences,University of Delaware

Capítulos

  • 00:05Título
  • 01:26Experimental Preparations
  • 04:00Collecting Sea Urchin Gametes
  • 06:12Rowing the Eggs
  • 07:40Microinjection of the Sea Urchin Zygotes
  • 11:13Injection of Reporter Constructs Does Not Affect Development
  • 12:05Conclusion

Summary

Tadução automática

Tadução automática

Mikroinjektion er en almindelig teknik, der anvendes til at levere DNA-konstruktioner, mRNA'er, morpholino antisense-oligonucleotider eller andre behandlinger i æg, embryoner, og celler fra forskellige arter.

Tags

MicroinjectionSea Urchin ZygotesHigh-throughputDNA ConstructsMRNAsRecombinant ProteinsGain Of FunctionLoss Of FunctionFluorescent DyeColorimetric DyeCell DeliveryEmbryo DeliveryBiological Processes

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