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High Throughput micro-injecties van Sea Urchin Zygotes

JoVE Journal
Biologia

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JoVE Journal Biologia

High Throughput Microinjections of Sea Urchin Zygotes

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High Throughput micro-injecties van Sea Urchin Zygotes

DOI:

10.3791/50841-v

•

12:40 min

•

January 21, 2014

•

Nadezda A. Stepicheva, Jia L. Song

¹Department of Biological Sciences,University of Delaware

Capítulos

00:05Título
01:26Experimental Preparations
04:00Collecting Sea Urchin Gametes
06:12Rowing the Eggs
07:40Microinjection of the Sea Urchin Zygotes
11:13Injection of Reporter Constructs Does Not Affect Development
12:05Conclusion

Summary

Tadução automática

Microinjectie is een veelgebruikte techniek gebruikt om DNA constructen mRNA, morfolino antisense oligonucleotiden of andere behandelingen te leveren in eieren, embryo's en cellen van verschillende soorten.

Tags

Keywords: Microinjection Sea Urchin Zygotes High-throughput DNA Constructs MRNAs Recombinant Proteins Gain Of Function Loss Of Function Fluorescent Dye Colorimetric Dye Cell Delivery Embryo Delivery Biological Processes

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