É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:11Prepare Protein-only Amyloid Directly from Native Proteins
- 02:06Prepare Hybrid Amyloid from Stabilized Soluble Oligomers of Native Proteins
- 04:01Results: Analysis of the Amyloid and Soluble Protein Oligomer
- 05:25Conclusion
Proteins can either adopt a native structure or misfold into insoluble amyloid. Conditions that favor the misfolding pathway lead to the formation of different types of amyloid fibrils. The methods described here allow rapid conversion of native proteins into amyloid in vitro.
