Journal

Biologia

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

DOI:

10.3791/50894-v

•

13:40 min

•

February 17, 2014

•

Alice Verchère, Manuela Dezi, Isabelle Broutin, Martin Picard

¹Laboratoire de Cristallographie & RMN Biologiques,CNRS & Université Paris Descartes

Capítulos

00:05Título
03:53Protein Production and Purification
05:24Preparation of Proteoliposomes
08:35Assessment of the Reconstitution Efficacy on Sucrose Gradient
09:37Fluorescence Staining
10:28Monitoring Pyranine Fluorescence as a Function of Time While a Proton Gradient is Generated
12:40Conclusion

Summary

Tadução automática

We present a protocol for the in vitro investigation of efflux pumps from Pseudomonas aeruginosa. This protocol allows for the generation of a robust, reversible, and tunable proton gradient within the liposome membrane and hence should be adaptable to any membrane protein energized by the protomotive force.

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