É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Department of Surgery, Davis Heart and Lung Research Institute,The Ohio State University Wexner Medical Center, 2Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute,The Ohio State University Wexner Medical Center, 3Department of Molecular Biophysics and Physiology,Rush University Medical Center, 4Department of Internal Medicine, Davis Heart and Lung Research Institute,The Ohio State University Wexner Medical Center
Capítulos
- 00:05Título
- 01:29Setting Up Osmotic-stress Perfusion System and Solution Preparation
- 02:00Preparing Intact Single Flexor Digitorum Brevis (FDB) Muscle Fibers from Mouse
- 05:12Fluo-4 AM Dye Loading and Ca2+ Imaging (Sparks Measurement)
- 07:39Data Analysis
- 08:14Results: Ca2+ Sparks in Young, Aged, and Diseased Mice
- 10:28Conclusion
