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观察和量化纤维细胞介导的纤维素凝胶压实

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction

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观察和量化纤维细胞介导的纤维素凝胶压实

DOI:

10.3791/50918-v

•

10:37 min

•

January 16, 2014

•

Aribet M. De Jesús, Edward A. Sander

¹Department of Biomedical Engineering,University of Iowa

Capítulos

00:05Título
01:42Fibrin Gel Preparation
04:41Cell Explant Preparation
07:42Time-lapse Imaging and Strain Tracking
09:23Results: Time-lapse Microscopy and Strain Tracking of Fibrin Reorganization
09:58Conclusion

Summary

Tadução automática

延时显微镜和图像处理技术用于观察和分析成纤维细胞介导凝胶压实和纤维素纤维在48小时内在环境控制的生物反应器中的重新调整。

Tags

Fibrin Gel Compaction Fibroblast-mediated Contraction Gel Microstructure Reorganization Time-lapse DIC Microscopy Strain Mapping Mechanobiology Cell-matrix Interactions Wound Healing

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