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Establishment of a Surgically-induced Model in Mice to Investigate the Protective Role of Progranulin in Osteoarthritis
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Bioengenharia
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JoVE Journal Bioengenharia
Establishment of a Surgically-induced Model in Mice to Investigate the Protective Role of Progranulin in Osteoarthritis

Establishment of a Surgically-induced Model in Mice to Investigate the Protective Role of Progranulin in Osteoarthritis

DOI:
10.3791/50924-v

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07:58 min

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February 25, 2014

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, ,
1Department of Orthopaedic Surgery,NYU Hospital for Joint Diseases, 2Department of Orthopaedic Surgery,New York University Medical Center

Capítulos

  • 00:05Título
  • 01:30Surgery
  • 04:06Histological Scoring of Surgical DMM Model
  • 06:19Results: Deficiency of PGRN Increases the Surgically-induced Osteoarthritis Development in DMM Mice
  • 07:32Conclusion

Summary

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We describe a protocol for the destabilization of the medial meniscus (DMM) model in mice, an effective tool for osteoarthritis (OA) research. In addition, we have demonstrated that deficiency of progranulincan exaggerate OA development and progression by using this model, indicating that progranulin plays a protective role in the pathogenesis of OA.

Tags

Keywords: OsteoarthritisDestabilization Of Medial Meniscus (DMM)Progranulin (PGRN)Surgically-induced ModelCartilage DegradationOsteophyte FormationGene Expression

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