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小鼠基因组工程使用Designer核酸酶
JoVE Journal
Biologia
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JoVE Journal Biologia
Mouse Genome Engineering Using Designer Nucleases
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小鼠基因组工程使用Designer核酸酶

DOI:
10.3791/50930-v

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12:04 min

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April 02, 2014

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, , ,
1Institute of Laboratory Animal Science,University of Zurich, 2Department of Genetics, Cell Biology & Development and Center for Genome Engineering,University of Minnesota

Capítulos

  • 00:05Título
  • 01:18Identifying TALEN Target Sites
  • 02:30Embryo Isolation
  • 04:23Embryo Microinjection
  • 06:33Embryo Transfer
  • 09:15Identifying Founders with Targeted Mutations
  • 11:21Conclusion

Summary

Tadução automática

Tadução automática

设计师核酸酶如锌指核酸酶（ZFN）和转录活化因子样效应核酸酶（TALENS）可用于通过触发两个非同源末端连接（NHEJ）和同源重组（HR）途径来修改小鼠早期胚胎的基因组。这些进展使快速生成小鼠的精确的遗传修饰。

Tags

Keywords: Mouse Genome EngineeringDesigner NucleasesZinc Finger Nucleases (ZFNs)Transcription Activator-like Effector Nucleases (TALENs)CRISPR/Cas9Site-specific Genome ModificationKnockoutKnock-inTransgenic MiceGenome EditingMicroinjectionFertilized Mouse Oocytes

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