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高通量流式细胞仪检测细胞为基础的试验检测抗体，以N-甲基-D-天冬氨酸受体或多巴胺2受体在人血清

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Medicina

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JoVE Journal Medicina

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

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高通量流式细胞仪检测细胞为基础的试验检测抗体，以N-甲基-D-天冬氨酸受体或多巴胺2受体在人血清

DOI:

10.3791/50935-v

•

10:19 min

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November 23, 2013

•

Mazen Amatoury, Vera Merheb, Jessica Langer, Xin Maggie Wang, Russell Clive Dale, Fabienne Brilot

¹Institute for Neuroscience and Muscle Research, The Kids Research Institute at the Children’s Hospital at Westmead,The University of Sydney, ²Flow Cytometry Centre,Westmead Millennium Institute for Medical Research

Capítulos

00:05Título
01:37Subcloning Strategy: D2R or NMDAR into pIRES2-EGFP Vector
02:20Transfection: Expression of Neuronal Antigen in HEK293 Cells
04:15Flow Cytometry Cell-based Assay for Detection of Antibody to Surface Neuronal Antigens
07:44Results: Detection/Analysis of Antibody Binding
09:45Conclusion

Summary

Tadução automática

在最近几年，活细胞检测已被成功地用于检测抗体的表面和构象的抗原。在这里，我们利用高通量流式细胞仪使的患者大样本的分析描述的方法。检测新抗体将提高诊断的免疫介导的病症和治疗。

Tags

Keywords: High-throughput Flow Cytometry Cell-based Assay Antibodies N-methyl-D-aspartate Receptor Dopamine-2 Receptor Human Serum Autoimmune CNS Diseases Neurotransmission Eukaryotic Cells Fluorochrome-conjugated Secondary Antibodies Quantitative High-throughput Sampler System Antigenic Targets Central Nervous System Immune-mediated Disorders

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