Journal
/
Ambiente
/
潜在土壤胞外酶活性的高通量荧光测量
JoVE Journal
Ambiente
This content is Free Access.
JoVE Journal Ambiente
High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
Please note that all translations are automatically generated. Click here for the English version.

潜在土壤胞外酶活性的高通量荧光测量

DOI:
10.3791/50961-v

•

12:33 min

•

November 15, 2013

•

, , , , ,
1Natural Resource Ecology Laboratory,Colorado State University, 2Biosciences Division,Oak Ridge National Laboratory, 3Department of Bioengineering,University of Colorado

Capítulos

  • 00:05Título
  • 02:18Assay Set-up
  • 04:00Incubation of Assay Plates
  • 04:56Fluorescent Measurements on a Microplate Fluorometer
  • 05:43Data Analysis
  • 07:03Results: Analysis of Extracellular Enzyme Activity (EEA)
  • 11:05Conclusion

Summary

Tadução automática

Tadução automática

测量土壤胞外酶活性的潜在利率，绑定到一个荧光染料的合成底物加入到土壤样品。酶的活性被测量为荧光染料从基板释放的酶催化反应，其中更高的荧光指示多底物降解。

Tags

Keywords: Soil Extracellular Enzyme ActivitiesFluorometric MeasurementSoil Microbial Enzyme ActivityFluorescence Enzyme AssaySoil SlurriesFluorescently Labeled SubstratesPotential Enzyme ActivitySoil Ecosystem Functional DynamicsEnzyme Kinetics

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/50961?article_type=v

在使用高通量微孔板测定水，土壤，沉积物和微生物测定胞外酶活性的
check_url/pt/50961?article_type=v

设计一个连续和操作<sup> 13</sup> C和<sup> 15</sup>Ñ贴标商会统一或差分，代谢和结构，植物同位素标记
check_url/pt/50961?article_type=v

土壤采样及昆虫病原线虫的分离（Steine​​rnematidae，异小）
check_url/pt/50961?article_type=v

从农业土壤使用静态钱伯斯温室气体流量测量
check_url/pt/50961?article_type=v

半高通量筛选潜在的抗干旱的生菜（<em>莴苣</em>）种质资源
check_url/pt/50961?article_type=v

一个插角Thermogradient表来控制土壤温度为评估植物生长和监测土壤过程
check_url/pt/50961?article_type=v

协议在草坪系统量化转换农药残留

3维 (3 d) 印制的高吞吐量斑马鱼胚胎排列模板
check_url/pt/50961?article_type=v

利用主成分扩展地形模型绘制土壤再分配和土壤有机碳
check_url/pt/50961?article_type=v

从环境样品中筛选高通量的安德森菌: 植物组织、散装土壤和根际土壤

Read Article