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Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
JoVE Journal
Biologia
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JoVE Journal Biologia
Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR

DOI:
10.3791/50967-v

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08:16 min

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February 01, 2014

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, ,
1Center for Food Safety and Applied Nutrition, Division of Molecular Biology,Food and Drug Administration

Capítulos

  • 00:05Título
  • 02:02Preparing Live and Dead Cell Mixtures for PMA Treatment
  • 03:16Treating Cells with PMA
  • 04:41qPCR
  • 05:47Results: Selective Detection of Live E. coli O157:H7 Cells by PMA-qPCR
  • 07:30Conclusion

Summary

Tadução automática

Tadução automática

A qPCR assay was developed for detection of Escherichia coli O157:H7 targeting a unique genetic marker, Z3276. The qPCR was combined with propidium monoazide (PMA) treatment for live cell detection. This protocol has been modified and adapted to a 96-well plate format for easy and consistent handling of numerous samples

Tags

Live E. Coli O157:H7 DetectionORF Z3276QPCR AssayPMA-qPCRLive Cell Detection96-well Plate FormatFood SafetyEnvironmental Monitoring

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