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Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity
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Biologia
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JoVE Journal Biologia
Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

DOI:
10.3791/50972-v

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04:47 min

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October 28, 2013

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, , , ,
1The Joint Institute for Biological Sciences,Oak Ridge National Laboratory, 2490 BioTech, Inc., 3The Center for Environmental Biotechnology,The University of Tennessee, Knoxville

Capítulos

  • 00:05Título
  • 01:11Cell Plating and Experimental Treatment
  • 02:22Imaging and Data Analysis
  • 03:06Results: Representative Cellular Bioluminescence Dynamics in Response to Chemical Exposure
  • 04:15Conclusion

Summary

Tadução automática

Tadução automática

Mammalian cells expressing the bacterial bioluminescence gene cassette (lux) produce light autonomously. The resulting bioluminescent dynamics upon chemical exposure have been demonstrated to reflect the treatment effects on cellular growth and metabolism, making these cells an inexpensive, continuous, real-time toxicity screening tool that can easily be adapted for high-throughput automation.

Tags

Autonomously BioluminescentMammalian CellsCytotoxicity MonitoringLux Gene CassetteReal-timeNon-destructiveIn Vitro AssayToxicological StudiesCell-based AssayOptical SignalBiosentinelCellular Metabolism

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