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Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
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Neurociência
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JoVE Journal Neurociência
Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

DOI:
10.3791/50989-v

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11:32 min

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November 12, 2013

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, , , ,
1Wolfson Centre for Age-Related Diseases,King’s College London, 2MRC Centre for Developmental Neurobiology,King’s College London

Capítulos

  • 00:05Título
  • 01:19Dissection and Dissociation of Rat RMS Neuroblasts
  • 04:30Nucleofection
  • 06:43Embedding
  • 08:50Results: Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
  • 11:09Conclusion

Summary

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Neuroblast migration is a crucial step in postnatal neurogenesis. The protocol described here can be used to investigate the role of candidate regulators of neuroblast migration by employing DNA/small hairpin RNA (shRNA) nucleofection and a 3D migration assay with neuroblasts isolated from the rodent postnatal rostral migratory stream.

Tags

Keywords: Subventricular ZoneNeuroblast MigrationRostral Migratory StreamOlfactory BulbNeurogenesisNucleofection3D In Vitro Migration AssayPlasmid DNAShRNASiRNAReaggregationSignaling Pathways

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