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Brug mikrofluidik Chips til Live Imaging og Undersøgelse af Injury Responses i<em> Drosophila</em> Larver
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1Department of Molecular, Cellular and Developmental Biology,University of Michigan, 2Department of Biomedical Engineering,University of Michigan, 3Life Sciences Institute,University of Michigan, 4Department of Cell and Developmental Biology,University of Michigan, 5Department of Mechanical Engineering,University of Michigan
Capítulos
- 00:05Título
- 01:47Preparing the PDMS Chip
- 03:47Using the Larva Chip for Live Imaging
- 07:23Inducing a Nerve Crush Injury to Larval Segmental Nerves
- 08:42Immobilization Enhanced Imaging
- 10:25Conclusion
Drosophila larver er en attraktiv model for billeddannelse på grund af deres gennemskinnelige neglebånd og kraftfulde genetik. Denne protokol beskriver, hvordan at udnytte en enkelt-lags PDMS-enhed, kaldet 'larve chip' for levende billeddannelse af cellulære processer indenfor neuroner i 3. stadie Drosophila larver.
