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Bruke Microfluidics Chips for Live Imaging and Study of Injury Responses i<em> Drosophila</em> Larver
This content is Free Access.
1Department of Molecular, Cellular and Developmental Biology,University of Michigan, 2Department of Biomedical Engineering,University of Michigan, 3Life Sciences Institute,University of Michigan, 4Department of Cell and Developmental Biology,University of Michigan, 5Department of Mechanical Engineering,University of Michigan
Capítulos
- 00:05Título
- 01:47Preparing the PDMS Chip
- 03:47Using the Larva Chip for Live Imaging
- 07:23Inducing a Nerve Crush Injury to Larval Segmental Nerves
- 08:42Immobilization Enhanced Imaging
- 10:25Conclusion
Drosophila larver er et attraktivt modellsystem for levende avbildning på grunn av deres gjennomskinnelig skjellaget og kraftige genetikk. Denne protokollen beskriver hvordan du kan utnytte en single-layer PDMS enhet, kalt 'larve chip "for levende avbildning av cellulære prosesser i nevroner av 3 rd instar Drosophila larver.
