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对于I型胶原蛋白的制备皮肤方法的改进

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

An Improved Method for the Preparation of Type I Collagen From Skin

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对于I型胶原蛋白的制备皮肤方法的改进

DOI:

10.3791/51011-v

•

05:17 min

•

January 21, 2014

•

Christina A. Pacak², Allison A. MacKay, Douglas B. Cowan²

¹Department of Anesthesiology, Perioperative and Pain Medicine,Boston Children’s Hospital, ²Department of Anesthesia,Harvard Medical School

Capítulos

00:05Título
01:10Preparing the Dermal Sample
01:44Removing Noncollagenous Solubilized Material
02:48Type I Collagen Extraction
04:22Results: Rapid Isolation from Dermis to Yield COL1 Polymerized into Fibrils
04:58Conclusion

Summary

Tadução automática

对于可溶性Ⅰ型胶原（COL1）隔离的传统程序需要从开始10天左右，由于长时间的缓冲液孵育和纤丝费力再悬浮完成。在这里，我们描述了从真皮小活检净化COL1，在不到3小时的方法。

Tags

Keywords: Type I Collagen COL1 Collagen Extraction Skin-derived Collagen Autologous Collagen Centrifugal Filtration Regenerative Applications Clinical Applications Cosmetic Surgery Dermal Injections Bone Grafting Reconstructive Surgery

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