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模拟胰腺神经可塑性：<em>体外</em>双神经元可塑性分析

JoVE Journal
Medicina

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JoVE Journal Medicina

Simulating Pancreatic Neuroplasticity: In Vitro Dual-neuron Plasticity Assay

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模拟胰腺神经可塑性：<em>体外</em>双神经元可塑性分析

DOI:

10.3791/51049-v

•

10:29 min

•

April 14, 2014

•

Ihsan Ekin Demir, Elke Tieftrunk, Karl-Herbert Schäfer, Helmut Friess, Güralp O. Ceyhan

¹Department of Surgery, Klinikum rechts der Isar,Technische Universität München, ²Department of Biotechnology,University of Applied Sciences Kaiserslautern/Zweibrücken

Capítulos

00:05Título
01:47Collecting Tissue Extracts and Cell Line Supernatants
03:48Isolation of the Dorsal Root Ganglia (DRG) Neurons
05:35Culturing Neurons and Glia with Pancreatic Tissue Extracts and Cell-line Supernatants
08:01Results: Neuronal and Glial Plasticity Due to Pancreatic Microenvironmental Cues
09:37Conclusion

Summary

Tadução automática

神经元可塑性是一个日益认识到，但是不能充分理解的胃肠（GI）道的特征。这里，在人胰腺疾病的实例中，我们提出了对神经元可塑性的在胃肠道中在两个形态和功能水平的研究在体外神经可塑性试验。

Tags

Pancreatic Neuroplasticity Enteric Nervous System Gastrointestinal Innervation Pancreatic Cancer Chronic Pancreatitis Dorsal Root Ganglia Myenteric Plexus Dual-neuron Assay In Vitro Model Neuronal Morphology Electrophysiology Microenvironmental Modulation

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