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Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

DOI:
10.3791/51052-v

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07:03 min

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January 31, 2014

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, , ,
1Department of Bioengineering,University of Utah, 2Department of Radiology and Radiological Science,Johns Hopkins University School of Medicine, 3Institute for NanoBiotechnology,Johns Hopkins University

Capítulos

  • 00:05Título
  • 01:25Photo-activation and IV Injection of Caged Collagen Mimetic Peptides (CMPs)
  • 02:22Near Infrared (NIR) Fluorescence Imaging of Skeletal Collagen Remodeling Activity In vivo
  • 03:31Visualization of Collagens in Ex vivo Tissue Sections
  • 05:19Representative Images
  • 06:28Conclusion

Summary

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This procedure demonstrates in vivo near IR fluorescence imaging of collagen remodeling activities in mice as well as ex vivo staining of collagens in tissue sections using caged collagen mimetic peptides that can be photo-triggered to hybridize with denatured collagen strands.

Tags

CollagenDenatured CollagenExtracellular MatrixCaged Collagen Mimetic PeptidePhoto-triggered HybridizationIn Vivo ImagingEx Vivo ImagingProteasesTriple Helix

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