Journal
/
Biologia
/
Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy

Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy

DOI:
10.3791/51074-v

•

11:43 min

•

May 10, 2014

•

, , , ,
1Institute of Molecular Biology and Genetics and Interdisciplinary Nanoscience Center,Aarhus University, 2Departments of Chemistry and Physics,McGill University

Capítulos

  • 00:05Título
  • 01:15Import the Image Sequence of Interest into an Imaging Analysis Program
  • 02:37Perform the Analysis
  • 06:52Calculate Average of the Diffusion Coefficient
  • 07:56Results: Example ROI Crops, Diffusion, and k2 Plots
  • 11:09Conclusion

Summary

Tadução automática

Tadução automática

This paper provides a step by step guide to the fluctuation analysis technique k-Space Image Correlation Spectroscopy (kICS) for measuring diffusion coefficients of fluorescently labeled plasma membrane proteins in live mammalian cells.

Tags

Diffusion CoefficientsEGFPPlasma Membrane ProteinsK-Space Image Correlation Spectroscopy (kICS)Fluorescence MicroscopyImage AnalysisAquaporin-3Membrane Protein Diffusion

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry

Crystallization of Membrane Proteins in Lipidic Mesophases

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins

Imaging Plasma Membrane Deformations With pTIRFM

Using Digital Image Correlation to Characterize Local Strains on Vascular Tissue Specimens

Read Article