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绿色荧光蛋白标记的质膜蛋白质的扩散系数的简易测量采用K空间图像相关光谱

JoVE Journal
Biologia

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JoVE Journal Biologia

Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy

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绿色荧光蛋白标记的质膜蛋白质的扩散系数的简易测量采用K空间图像相关光谱

DOI:

10.3791/51074-v

•

11:43 min

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May 10, 2014

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Eva C. Arnspang, Jennifer S. Koffman, Saw Marlar, Paul W. Wiseman, Lene N. Nejsum

¹Institute of Molecular Biology and Genetics and Interdisciplinary Nanoscience Center,Aarhus University, ²Departments of Chemistry and Physics,McGill University

Capítulos

00:05Título
01:15Import the Image Sequence of Interest into an Imaging Analysis Program
02:37Perform the Analysis
06:52Calculate Average of the Diffusion Coefficient
07:56Results: Example ROI Crops, Diffusion, and k² Plots
11:09Conclusion

Summary

Tadução automática

本文提供了一个一步一步引导到波动分析技术K空间图像相关光谱（KICS），用于测量扩散系数的荧光在活的哺乳动物细胞标记质膜蛋白。

Tags

Keywords: Diffusion Coefficients EGFP Plasma Membrane Proteins K-Space Image Correlation Spectroscopy (kICS)Fluorescence Microscopy Image Analysis Aquaporin-3 Membrane Protein Diffusion

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