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自动分离<em> C。线虫</em>可多段由细菌病原体殖民者

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen

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自动分离<em> C。线虫</em>可多段由细菌病原体殖民者

DOI:

10.3791/51090-v

•

11:18 min

•

March 21, 2014

•

Kwame Twumasi-Boateng, Maureen Berg, Michael Shapira

¹Department of Integrative Biology,University of California, Berkeley

Capítulos

00:05Título
01:49Infection of Synchronized Young Adult Worms with Pseudomonas aeruginosa
04:44Setting up the Wormsorter
06:19Sorting Colonized vs Noncolonized Worms
08:28Results: Colonization is Not Required for the Transcriptional Response to P. aeruginosa
10:19Conclusion

Summary

Tadução automática

该wormsorter通过根据荧光记者表达分拣蠕虫有利于遗传筛选在秀丽隐杆线虫 。在这里，我们描述了一个新的使用：根据定植通过GFP表达病原体排序，我们用它来检查病原体识别在启动免疫反应的了解甚少的作用。

Tags

C. Elegans Caenorhabditis Elegans Bacterial Pathogen Wormsorter FACS GFP Pseudomonas Aeruginosa Immune Response Pathogen Colonization Pathogen-associated Molecular Patterns Damage Detection

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