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人体前庭神经鞘瘤的原代培养

JoVE Journal
Medicina

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JoVE Journal Medicina

Primary Culture of Human Vestibular Schwannomas

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人体前庭神经鞘瘤的原代培养

DOI:

10.3791/51093-v

•

10:50 min

•

July 20, 2014

•

Nathan M. Schularick, J. Jason Clark, Marlan R. Hansen

¹Department of Otolaryngology-Head and Neck,University of Iowa Hospitals and Clinics

Capítulos

00:05Título
01:31Setup: The Day Before Tumor Harvest
02:21Setup and Tumor Harvest
04:08Tissue Dissociation and Trituration
07:59Cell Plating
09:19Results: Primary Vestibular Schwannoma Cultures
10:18Conclusion

Summary

Tadução automática

前庭神经鞘瘤（VSS）是雪旺细胞（SC）的原点非恶性肿瘤，与在NF2的肿瘤抑制基因的突变相关联。我们提出一个可重复的，高效的协议，用于原代人类VS细胞培养，允许分子和细胞实验操作和分析，概括人类疾病的异质性。

Tags

Keywords: Vestibular Schwannoma Schwann Cell Tumor Neurofibromatosis Type 2 NF2 Tumor Suppressor Gene Primary Cell Culture HEI193 Cell Line HPV E6 And E7 Oncogenes Tumorigenesis

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