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Biologia
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Identification of Post-translational Modifications of Plant Protein Complexes
JoVE Journal
Biologia
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JoVE Journal Biologia
Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

DOI:
10.3791/51095-v

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10:07 min

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February 22, 2014

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, , , , ,
1School of Life Sciences,University of Warwick, 2The Sainsbury Laboratory,Norwich Research Park, 3Research School of Biology,The Australian National University

Capítulos

  • 00:05Título
  • 01:37N. benthamiana and A. thaliana Protein Samples
  • 03:24Protein Immunoprecipitation
  • 05:32Protein Digestion
  • 07:48Results: Identification of Post-translational Modifications of Tagged Proteins
  • 09:13Conclusion

Summary

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Tadução automática

We describe here a protocol for the purification and characterization of plant protein complexes. We demonstrate that by immunoprecipitating a single protein within a complex, so we can identify its post-translational modifications and its interacting partners.

Tags

Keywords: Plant ProteinsPost-translational ModificationsPTMsProtein ComplexesImmune ComplexesNB-LRR ProteinsMass SpectrometryProtein PurificationImmunoprecipitation

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