Journal
/
/
植物蛋白复合体的翻译后修饰的鉴定
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Identification of Post-translational Modifications of Plant Protein Complexes
DOI:

10:07 min

February 22, 2014

, , , , ,

Capítulos

  • 00:05Título
  • 01:37N. benthamiana and A. thaliana Protein Samples
  • 03:24Protein Immunoprecipitation
  • 05:32Protein Digestion
  • 07:48Results: Identification of Post-translational Modifications of Tagged Proteins
  • 09:13Conclusion

Summary

Tadução automática

我们在这里描述的一个协议,用于植物蛋白质复合物的纯化和表征。我们证明了在一个复杂的免疫沉淀一个单一的蛋白质,这样我们就可以识别其翻译后修饰及其相互作用的合作伙伴。

Vídeos Relacionados

Read Article