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成像生物膜损伤和参与亚细胞修复过程

JoVE Journal
Biologia

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JoVE Journal Biologia

Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair

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成像生物膜损伤和参与亚细胞修复过程

DOI:

10.3791/51106-v

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16:44 min

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March 24, 2014

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Aurelia Defour*¹, S. C. Sreetama*¹, Jyoti K. Jaiswal²

¹Center for Genetic Medicine Research,Children’s National Medical Center, ²Department of Integrative Systems Biology,George Washington University

Capítulos

00:05Título
01:53Imaging Cell Membrane Repair: Bulk Wounding
04:59Live Imaging: Kinetics of Cell Membrane Repair
06:23Imaging Bulk Injury Induced Lysosomal Exocytosis
09:18Live Imaging of Cell Membrane Injury Triggered Subcellular Trafficking
10:52Results: Single Cell Imaging of Injury and Repair
15:58Conclusion

Summary

Tadução automática

愈合损伤的细胞的过程中涉及到贩卖特异性蛋白和亚细胞区室中的细胞膜损伤的部位。本协议描述分析，以监测这些进程。

Tags

Keywords: Cell Membrane Injury Cell Membrane Repair Cell Healing Subcellular Processes Lysosomal Exocytosis TIRF Microscopy Pulsed Laser Injury Cell Culture End-point Based Assay Real-time Imaging

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