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An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy
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JoVE Journal Medicina
An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy

An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy

DOI:
10.3791/51108-v

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09:52 min

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April 21, 2014

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, , ,
1Developmental Biology Institute of Marseille,Aix Marseille University, 2European Research Center for Medical Imaging, Campus de la Timone, 3Vesalius Research Center,KU Leuven Campus Gasthuisberg

Capítulos

  • 00:05Título
  • 01:26Craniotomy
  • 03:48Spheroid Preparation and Implantation
  • 04:58Closing the Craniotomy with a Window
  • 07:59Tissue Imaging
  • 09:20Conclusion

Summary

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Tadução automática

We have established a cortical orthotopic glioblastoma model in mice for intravital two-photon microscopy that recapitulates the biophysical constraints normally at play during the growth of the tumor. A chronic glass window replacing the skull above the tumor enables the follow-up of the tumor progression over time by two-photon microscopy.

Tags

GlioblastomaMouse ModelOrthotopicTwo-photon MicroscopyBrain ParenchymaTumor SpheroidDextran GelCraniotomyFluorescent TransgenicIntravital ImagingNeuro-oncologyDrug Testing

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