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Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method
JoVE Journal
Biologia
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JoVE Journal Biologia
Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method

Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method

DOI:
10.3791/51111-v

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09:38 min

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October 23, 2013

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, ,
1Biochemistry and Molecular Biology,Michigan State Universtiy

Capítulos

  • 00:05Título
  • 01:51Solution Preparation and Phloem Exudate Collection
  • 05:06Results: Analysis of Phloem Exudates
  • 08:17Conclusion

Summary

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Knowledge of the composition of the phloem sap as well as the mechanism of its loading and long-distance transport is essential for the understanding of long-distance signaling in plant development and stress/pathogen response and of assimilate transport. This manuscript describes the collection of phloem exudates using the EDTA-facilitated method.

Tags

Keywords: Phloem ExudateEDTA-facilitated MethodArabidopsisPhloem SapPhloem CompositionPhloem AnalysisPhloem TransportPlant DevelopmentStress ResponseMetabolite AnalysisProtein AnalysisRNA AnalysisPlant SpeciesTeaching Laboratory

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