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Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

JoVE Journal
Biologia

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JoVE Journal Biologia

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

DOI:

10.3791/51138-v

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06:30 min

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February 05, 2014

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Lingyan Xing^2,3, Tyler S. Quist, Tamara J. Stevenson, Timothy J. Dahlem, Joshua L. Bonkowsky^2,3,5

¹Division of Pediatric Neurology, Department of Pediatrics,University of Utah School of Medicine, ²Department of Neurobiology and Anatomy,University of Utah School of Medicine, ³Interdepartmental Program in Neurosciences,University of Utah School of Medicine, ⁴Mutation Generation and Detection Core, HSC Core Research Facility,University of Utah School of Medicine, ⁵Department of Neurology,University of Utah School of Medicine

Capítulos

00:05Título
01:09DNA Preparation
02:24PCR Settings
03:24PCR Analysis by HRMA
04:31PCR Identified Mutations
06:06Conclusion

Summary

Tadução automática

PCR combined with high-resolution melt analysis (HRMA) is demonstrated as a rapid and efficient method to genotype zebrafish.

Tags

Keywords: Zebrafish Genotyping PCR High-resolution Melt Analysis HRMA Genetic Tools Mutation Transgenic Screening Model System Development Disease Drug Screening Rapid Efficient Sensitive Inexpensive Contamination

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