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Hurtig Syntese og Screening af kemisk aktiveret transkriptionsfaktorer med GFP-baserede Journalister
JoVE Journal
Biologia
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JoVE Journal Biologia
Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Hurtig Syntese og Screening af kemisk aktiveret transkriptionsfaktorer med GFP-baserede Journalister

DOI:
10.3791/51153-v

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09:22 min

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November 26, 2013

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, , ,
1The Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Department of Molecular Biology,Princeton University, 3Division of Chemistry and Chemical Engineering,California Institute of Technology

Capítulos

  • 00:05Título
  • 01:33Creation of Artificial Transcription Factors (ATFs)
  • 02:28Creation of ATF Plasmid Reporter
  • 03:57Quantify the Effect of ATF Induction on Gene Expression Using GFP Reporter
  • 05:33Quantify the Effect of the ATF Induction on Cell Growth
  • 06:42Create Inducible Genomic Alleles
  • 07:20Results: Analysis of GFP Induction by ATF-promoter Pairs
  • 08:39Conclusion

Summary

Tadução automática

Tadução automática

Denne protokol beskriver en eksperimentel procedure for hurtig opbygning af kunstige transkriptionsfaktorer (ATFS) med beslægtede GFP journalister og kvantificering af ATFS evnen til at stimulere GFP udtryk via flowcytometri.

Tags

Keywords: Synthetic BiologyTranscription FactorsGene ExpressionReporter SystemsGFPHomologous RecombinationArtificial Transcription FactorsConditional Gene Expression

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