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Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

DOI:
10.3791/51161-v

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06:29 min

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January 29, 2014

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, ,
1Department of Immunopathology, Sanquin Research and Landsteiner Laboratory Academic Medical Center,University of Amsterdam, 2Department of Hematology, Academic Medical Center,University of Amsterdam

Capítulos

  • 00:05Título
  • 01:21Complement Deposition Analysis and Quantification of Hemolysis
  • 04:34Results: Quantitative Detection of Antibody-induced Complement Activation on RBCs
  • 06:02Conclusion

Summary

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Here we describe two assays for measuring complement activation induced by antibodies against red blood cells. The major advantage over the current assays is their quantitative and easy-to-interpret nature.

Tags

Keywords: AntibodyComplement ActivationRed Blood CellsRBCAlloantibodiesAutoantibodiesAutoimmune Hemolytic AnemiaAIHACoombs TestHemolytic AssayC3 DepositionC4 DepositionFACS AnalysisQuantitative Hemolytic AssaySpectrophotometryHemoglobin

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